Cole-Parmer QPCR-500 Real-Time qPCR System

SKU : 93947-00
The QPCR-500 Real-Time PCR System is a high specification, economically priced real time thermal cycler that accommodates a unique 48-well polypropylene PCR plate utilizing the same geometry as standard 384-well plates, but only 1/8 of the size. This enables users to dramatically reduce the qPCR reagent volumes compared to traditional 96-well instruments, whilst still producing a strong fluorescence signal. Minimizing the plate size also significantly improves thermal uniformity. A minimum volume of 5 µl is validated, cutting reagent costs in half and making more efficient use of expensive and hard to acquire template DNA samples. MIQE compliant open license software gives confidence when it comes to publishing your data. Eco48 allows for simple multiplex analysis with up to four dyes at once. Fast cycling enables several experiments per day, all at an economical price. The PCR system is an open platform that can utilize any chemistry, dye or PCR reagent. The easy to use software, makes analysis simple and fast. The system comes with two distinct softwares. The first drives the unit's set up and experimental runs while Eco Study is the analysis package. The software is open license, you can install them on as many PCs as you like, and MIQE compliant. With the QPCR-500 system and software, data collection is monitored in real time, allowing researchers to access run viability immediately. The user-friendly data analysis interface also allows researchers to easily view the amplification plot, melt curve analysis, and the analyzed results, including Cq values, PCR efficiency, R2 and Y-intercept.

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Specification

Includes
Dock and squeegee, EcoControl and EcoStudy software, USB stick, 50 PCR plates, 50 plate seals, Ethernet cable, and power cord.

Key Features

  • HRM functionality as standard with the ability to discriminate class IV SNP >99% of the time
  • Allows for simple multiplex analysis with up to four dyes at once
  • World's most thermally accurate block gives consistent data every time
  • High uniformity provides high quality data
  • Fast cycling enables several experiments per day, all at an economical price
  • Fastest block-based real-time PCR system: 40 cycles in 40 minutes as standard and 40 cycles in as little as 15 minutes after optimization of temperatures and hold times
  • Open platform can utilize any chemistry, dye or PCR reagent
  • Easy to use software, making analysis simple and fast
  • Provides a small 34.5cm x 31cm (13.6” × 12.2”) footprint to save bench space
  • Research Use Only


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